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KMID : 0903620040450040207
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Journal of the Korean Society for Horticultural Science
2004 Volume.45 No. 4 p.207 ~ p.211
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Genetic Characterization of the Interspecific Asian Pears (Pyrus pyrifolia × P. ussuriensis) Using Molecular Markers Linked to Fruit Characteristics
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Kim Dae-Il
Hwang Jeong-Hwan
Lee Dong-Hoon
Shin Yong-Uk
Lee Hee-Jae
Choi Jun-Kyu
Shin Il-Seob
Kang Sang-Jo
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Abstract
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To develop molecular markers linked to the major fruit characteristics, skin color, grit cell content, firmness, core size, and weight were assessed and genetically analyzed in 135 F1 interspecifically hybridized progeny population from the cross between ‘Niitaka’ (Pyrus pyrifolia Nakai) and Suhyangri (P. ussuriensis Maxim.). All the characteristics except the amount of grit cells were showed a normal distribution. To detect molecular markers linked to several fruit characters, high quality genomic DNAs were extracted and sorted into two DNA pools by bulked segregant analysis. We selected two random amplified polymorphic DNA (RAPD) and eight amplified fragment length polymorphism (AFLP) markers. As the results through RAPD analysis, OPD05-850 and OPD08-500 were selected linked to fruit firmness and skin color, respectively. Eight AFLP markers through the 64 primer combinations were selected. Those were EaacMcag-440 and EaggMcag-470 linked to skin color; EacaMcac-259, EacaMctt-330, and EagcMcta-170 linked to grit cell amount; EaacMctg-350 linked to fruit firmness; EacaMctt-340 and EaagMctg-330 linked to fruit shape.
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KEYWORD
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amplified fragment length polymorphism, breeding efficiency, bulked segregant analysis, random amplified polymorphic DNA
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